Plasmid_Backbone
pPICZ^

Part:BBa_K3842005:Design

Designed by: Xiaoyi Feng   Group: iGEM21_GA_State_SW_Jiaotong   (2021-10-17)


pPICZαA(plasmid_backbone)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1215
    Illegal XbaI site found at 1235
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1215
    Illegal NotI site found at 1221
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1215
    Illegal BglII site found at 1
    Illegal BamHI site found at 1641
    Illegal XhoI site found at 1191
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1215
    Illegal XbaI site found at 1235
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1215
    Illegal XbaI site found at 1235
    Illegal NgoMIV site found at 2399
    Illegal NgoMIV site found at 2460
    Illegal AgeI site found at 1360
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1624
    Illegal BsaI.rc site found at 1823
    Illegal BsaI.rc site found at 2821


Design Notes

SPECIFIC care of mutation was needed


Source

https://www.snapgene.com/resources/plasmid-files/?set=yeast_plasmids&plasmid=pPICZ(alpha)_A

References